Simultaneous extraction of phycobiliproteins and carbonic Anhydrase from Spirulina Platensis Leb-52

Abstract

C-phycocyanin (C-PC), allophycocyanin (APC) and carbonic anhydrase (CA) are intracellular bioproducts produced by Spirulina platensis LEB-52. In order to extract them, a cell rupture step is required. The influence of extraction time on ultrasound-assisted extraction (UAE) was evaluated at two biomass concentrations (0.2 and 23 g/L). At biomass concentration of 0.2 g/L, 12-min UAE was sufficient to simultaneously obtain phycobiliproteins (C-PC and APC) and esterase-expressed CA. Enzyme activity of hydratase-expressed CA in cyanobacteria is very low. Therefore, to determine the activity of hydratase-expressed CA, biomass concentration of 23 g/L and 8-min extraction time were needed. UAE is an efficient method of rapid determination of phycobiliproteins in wet biomass, even though it is not a selective method which extracts an array of contaminants from biomass.